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The Comparative Study on Anti-inflammatory Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Ligustici Rhizoma
HwangBo, Min; Seo, Hyung-Sik
Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Ligustici Rhizoma (LR) extracted with two kinds of solvents, ethanol and distilled water. Methods : It is prepared two kinds of LR extracts 20, 50, 100 by first. MTT assay way to measure cytotoxicity is formed in Raw 264.7 cell. The anti-inflammation effect is measured by ability to inhibit production of NO in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. LR (20 ) extracted with ethanol was showed 80% cytotoxicity, LR (50 ) extracted with ethanol and LR (20, 50 ) extracted with water were showed 70% cytotoxicity, LR (100 ) extracted with ethanol and LR (100 ) extracted with water were showed 60% cytotoxicity in Raw 264.7 cell. 2. LR (100 ) extracted with ethanol was showed more significantly inhibitory effect on NO production than the water extraction. 3. Two kinds of LR extraction groups did not show significantly scavenging effect of DPPH radicals. 4. Two kinds of LR extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds of LR extracts have not cytotoxicity, statistically significant ability to scavenge DPPH radicals and effect to inhibit Propionibactrium acnes. LR extracted with ethanol only have a little effect to inhibit NO production. This study proposes that LR extracted with ethanol is more effective in anti-inflammation.
Ligustici Rhizoma; Extration Solvent; Antiinflammatory; Antioxidant. Anti-bacterial effect
Open Access
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © 2014 Journal of Pharmacopuncture. All rights reserved.