Sim, Jae-Young; Jo, Hyun-Chol; Lee, Seong-No; Kim, Kee-Hyun

The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide()-induced expressions of cyclooxygenase-2(COX-2), p38, jun N-terminal Kinase(JNK) and extra-signal response kinase(ERK) in RAW 264.7 cells, a murine macrophage cell line. Method : The expressions of COX-2, p38, JNK and ERK were determined by western blotting with corresponding antibodies. Results : 1. The 0.5, 1 and of bee venom inhibited significantly LPS and SNP-induced expression of COX-2 compared with control, respectively. The 0.5, 1 and of bee venom inhibited insignificantly -induced expression of COX-2 compared with control, respectively. 2. The 0.5, 1 and of bee venom inhibited significantly LPS, SNP and -induced expression of p38 compared with control, respectively. 3. The 1 and of bee venom inhibited significantly SNP-induced expression of JNK compared with control, respectively. All of bee venom inhibited insignificantly LPS and -induced expression of JNK compared with control, respectively. 4. The of bee venom inhibited significantly SNP-induced expression of ERK, the of bee venom increased significantly -induced expression of ERK compared with control. The 0.5, 1 and of bee venom inhibited insignificantly LPS-induced expression of ERK compared with control, respectively.

Bee venom; COX-2; P38; ERK; JNK; RAW 264.7 cell; LPS; SNP; H2O2

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.