Original Article

Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Eun-A Choi, Jeung-Won Yoon, Hak-Joo Choi, Dong-Hee Kim, Hwa-Seung Yoo,

Keywords: cytokine, GM-CSF, IL-1β, IL-6, lipopolysaccharide, TNF–α
DOI: http://dx.doi.org/10.3831/KPI.2012.15.1.007
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Aim:

Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods:

RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).

Results:

IS4 concentration inhibited the production of interleukin-1beta (IL-1β), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50㎍/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF–α) stimulated by LPS.

Conclusion:

IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.