Original Article
Differential Expression of Gene Profiles in MRGX-treated Lung Cancer
Yong-Kyun Kwon, Seung-Yeul Lee, Hwan-Soo Kang, Jung-Suk Sung, Chong-Kwan Cho, Hwa-Seung Yoo, Seungjin Shin, Jong-Soon Choi, Yeon-Weol Lee, Ik-Soon Jang,
Keywords: gene ontology, lung cancer, microarray, modified regular ginseng extract (MRGX)
DOI: http://dx.doi.org/10.3831/KPI.2013.16.018
Objectives:
Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles.
Methods:
To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network.
Results:
One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Conclusions:
Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.