Original article
Propugnating Effect of Bark of Rhizophora mucronata Against Different Toxicants Viz Carbon Tetrachloride, Ethanol and Paracetamol on HepG 2 Cell Lines
Jairaman Chitra, Yacoob Syed Ali Mohamed, Venkatraman Anuradha, Nagarajan Yogananth, Murugesan Gnanadesigan,
Keywords: cytotoxicity, ethanol: water (3:1) extract, toxicants, viable cells
DOI: http://dx.doi.org/https://doi.org/10.3831/KPI.2019.22.005
Objective:
The aim of the study was to evaluate the hepatoprotective activity of the bark extract (Ethanol: Water) in the ratio of (3:1) of Rhizophora mucronata (BERM) by intoxicating the HepG 2 cell lines with different toxicants viz, CCL 4 , Ethanol and Paracetamol with different concentrations of the extract were used. The HepG 2 cell lines were subjected to MTT Assay for studying the cytotoxicity.
Methods:
HepG 2 cells were plated using 96 well plate in 10% bovine serum, exposed to different toxicants viz, 2% CCl 4 , 60% Ethanol and 14 mM Paracetamol respectively. The various test concentrations (18.85, 37.5, 75, 150 and 300 μg/ml) of bark extract of Rhizophora mucronata was added and incubated for 24 hours. Medium was removed after incubation period and 0.5 mg/ml MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and again incubated for 4 hours at 37oC. Then MTT was removed the crystals was dissolved in DMSO and absorbance was measured at 570 nm.
Results:
The result showed that dose dependent increase in percentage of viability at the doses of 18.85, 37.5, 75, 150, 300 μg/ml. The results for the CCl4 intoxicated, at 300 μg/ml of the concentration of the extract, the % of viable cells was found out to be 99.6%, for Ethanol intoxicated, 97.67%, and Paracetamol induced, 75.37%, IC50 was 21.53 μg/ml, 12.61 μg/ml and 21.42 μg/ml respectively.