The Effects of Bee Venom on PLA2 and Calcium Concentration in Raw 264.7 Cells

Yun, Jong-Il; Jo, Hyun-Chul; Sim, Sung-Yong; Kim, Kee-Hyun

Keywords: Bee Venom; ; Calcium; RAW 264.7 cell

DOI: http://dx.doi.org/10.3831/KPI.2003.6.2.149

Objective : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide, sodium nitroprusside and hydrogen peroxide induced expression phospholipase and calcium concentration in RAW 264.7 cells, a murine macrophage cell line. Method : The expression of phospholipase was determined by western blotting with corresponding antibodies, and the generation of intracellular calcium concentration was investigated by delta scan system in RAW 264.7 cells. Results : 1. Compared with control, expressions of lipopolysaccharide-induced phospholipase were decreased significantly by of bee venom and decreased by 0.5, of bee venom. 2. Compared with control, expressions of sodium nitroprusside-induced phospholipase were decreased significantly by of bee venom but increased by 0.5, of bee venom. 3. Compared with control, expressions of hydrogen peroxide-induced phospholipase were decreased significaltly by of bee venom and decreased by of bee venom but increased by of bee venom. 4. Compared with control, lipopolysaccharide, sodium nitroprusside and hydrogen peroxide- induced intracellular calcium concentrations were decreased by 0.5, 1, of bee venom and by indomethacin