The Effects of Bee Venom on iNOS, TNF-α and NF-kB in RAW 264.7 Cells

Kim, Goon-Joong; Sim, Sung-Yong; Lee, Seong-No; Kim, Kee-Hyun;

Keywords: Bee Venom; iNOS; TNF-α; NF-kB; RAW 264.7 cell

DOI: http://dx.doi.org/10.3831/KPI.2003.6.2.045

Objective : The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-(TNF-) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line. Method : The expressions of expression iNOS and TNF- were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method. Results : 1. The 0.5, 1 and of bee venom on LPS-induced expression of iNOS, the of bee venom on SNP-induced expression of iNOS and the of bee venom on -induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and of bee venom inhibited significantly LPS and -induced expression of TNF- compared with control, respectively. The of bee venom increased significantly SNP-induced expression of TNF- compared with control. 3. The of bee venom on LPS-induced expression of NF-kB, the of bee venom on SNP-induced expression of NF-kB and the 0.5, of bee venom on -induced expression of NF-kB were inhibited significantly compared with control, respectively.