The Effects of t-butylhydroperoxide (tBHP) and Juglandis Semen on Brain Na+-Pump Activity

Kim, Dong-Hoon; Jang, Kyung-Jeon; Song, Choon-Ho; Ahn, Chang-Beohm;

DOI: http://dx.doi.org/10.3831/KPI.1999.2.1.013

This study was undertaken to determine whether Juglandis Semen (JAS) extraction exerts protective effect against oxidant-induced inhibition of -pump activity in cerebral cortex. -pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extraction. The oxygen consumption was increased by an increase in concentration from 5 to 100mM, concentration from 0.5 to 10 mM, and concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effct of tBHP and protective action of JAS on oxygen consumption. tBHP(1mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These result suggest that JAS exerts protective effect against tBHP-induced inhibition of -pump activity in the cerebral cortex, this effect may be due to by an antioxidant action