The effects of Bee Venom on NO, H2O2 in Raw 264.7 cells and IL-1 in D10S cells

Ha, Seang-Jong; Lee, Seong-No; Jo, Hyun-Chul; Kim, Kee-Hyun

Keywords: Bee Venom; NO; H2O2; IL-1; Raw 264.7 cell; D10S cell

DOI: http://dx.doi.org/10.3831/KPI.2002.5.2.052

Objectives : The purpose of this study was to investigate the effects of Bee Venom on NO, expression induced by LPS in Raw 264.7 cells as a murine marcrophage cell line and on IL-1 expression induced by LPS in D10S cells. Methods : The expression of NO was measured by MTT Assay and IL-1 by MTS Assay. The expression of was measured as ROS level within the cell using by FACS analysis. The non-toxic concentration(from ) of Bee Venom was determined by MTT Assay. Results : 1. Bee Venom inhibited the NO expression. The effective concentration of Bee Venom was after 3 hours, 1 and after 1 day and 2 days. The all concentration of Bee Venom inhibited the NO expression after 6, 12 hours and 3 days. 2. Bee Venom inhibited the expression in a dose-dependent manner compared to the control. 3. Bee Venom could not significantly inhibit the IL-1 expression.